Developing and validating assays that can be used to measure biomarkers in biological samples is crucial in biomarker discovery. Biomarkers are biological indicators of a particular disease or condition. They can be used to diagnose, monitor, and treat diseases, and also to assess the safety and efficacy of new drugs. The development of a new biomarker is a complex and challenging process. It requires a deep understanding of the biology of the disease or condition, as well as the ability to develop and validate an assay that can accurately measure the biomarker in biological samples.